NewsNatureFabrication of hybrid PCL-Chitosan/PVA nanofibers by hybrid electrospinning for Local melanoma skin cancer therapy
In this study, we have a type of functional nanofibers, hybrid nanofibers containing Polycaprolactone (PCL) / Chitosan (CS) and Polyvinyl alcohol (PVA) were successfully fabricated by hybrid electrospinning method. Pioglitazone Hydrochloride (PIO) was incorporated in PCL/CS solution due to its hydrophobic nature, and Gemcitabine Hydrochloride (GEM) was incorporated in PVA solution due to its hydrophilic nature. The prepared hybrid PCL-CS-PIO/PVA-GEM nanofibers exhibited a biphasic release profile in each case, indicating an initial burst followed by a sustained phase of GEM and PIO over 5 days. The maximum amount released from the hybrid nanofibers reached 75.88 ± 1.5% of the GEM and 64.54 ± 2.05% of the PIO in a medium of pH 7.4. Flow cytometry and MTT analyses demonstrated that the combination of PIO and GEM induced apoptosis and inhibited the growth of melanoma cancer cell lines by inhibiting proliferation with cell viability of 51.34 ± 2.2% and 46.04 ± 1.5% after 24 and 48 h compared to control group. Obtained nanofibers were assessed for their chemical composition and morphology followed by scanning electron microscopy (SEM), FTIR, contact angle measurements and drug release profile. Then, a cytotoxicity study was performed on human melanoma cancer cell line, followed by investigating its potential in treatment applications.
More from Cuppa
Apple's iPhone 18 goes on sale as customers line up at stores worldwideApple's iPhone 18 is now on sale worldwide, starting at $1,199 for the Pro model, with a new AI-powered Siri and upgraded camera features.
Are tea, coffee, red meat and alcohol giving you dangerous levels of iron?A new review finds that what you eat and drink, including tea, coffee, red meat and alcohol, can affect how much iron your body absorbs, which matters if you have iron overload.
NASA spacecraft discovers a huge new crater on the moonNASA found a massive new moon crater bigger than the Roman Colosseum, caused by an asteroid or comet impact two years ago that nobody noticed at the time.
Google's new 'CC' is an AI agent that helps families run their householdsGoogle has relaunched its CC AI agent as a household organiser for families, helping manage schedules, school emails, meal plans and shared to-do lists.
SpaceX to fly more NASA crews to space station under expanded $5.92 billion dealNASA has handed SpaceX a $946 million contract for three more astronaut missions to the International Space Station, extending the partnership through 2030.
Coffee on the nanoscale: Graphene oxide membrane removes half the caffeine while retaining key compoundsAustralian researchers at UNSW have developed a graphene oxide membrane that removes about half the caffeine from already-brewed coffee while keeping its flavour compounds intact.
China extending sea dominion to Batanes?China's Coast Guard is now patrolling waters east of the Philippines' Batanes islands, raising fears Beijing is expanding its maritime claims beyond the South China Sea.
Women and girls pay high price in DR Congo Ebola outbreakWomen and girls make up more than half of confirmed Ebola cases in the DR Congo outbreak, largely because they bear the burden of caring for the sick at home.